Western blot assays can provide semi-quantitative results but require expensive imaging systems to do so. ELISA ELISAs (Enzyme Linked Immuno-Sorbent Assays) are used to assess the strength of binding of antibodies (Kd measurements), signal transduction, quantitation of secreted proteins, and cell viability.ELISA (enzyme-linked immunosorbent assay) is a plate-based immunoassay used to detect antibodies or. Table 2 showed that data value distribution was tighter and the average standard error from the ELISA was much smaller than those of the Western blot, directly relating to the accuracy of the assay. ![]()
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